CRYSTALLINE PAPAIN: NUMBER AND REACTIVITY OF THIOL GROUPS; CHROMATOGRAPHIC BEHAVIOR
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چکیده
منابع مشابه
Crystalline Papain.
In an earlier study (1) of the amino acid composition of papain, it was assumed that all of the sulfur could be attributed to cysteine and cystine since methionine is absent from this protein. However, because of the sulfhydryl nature of this enzyme, the content of these amino acids is of some importance; we have used the method of Schram, Moore, and Bigwood (2) for this estimation. The results...
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1. Considerable differences were found between the rates and degrees of modification of native trout actin with iodo[2-(14)C]acetate and iodo[1-(14)C]acetamide. 2. With iodoacetate, G- and F-actin were both labelled in the N-terminal peptide only. This modification had little effect on the ability of the actin to polymerize. 3. Iodoacetamide labelled three cysteine residues in both G- and F-act...
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The reactivities of the two cysteine thiol groups of calf thymus F3 histone were investigated using 5,5'-dithiobis-[2- nitrobenzoic acid], (DTNB). In isolated histone, both thiol groups were available for reaction. However, analysis of reaction profiles of native deoxyribonucleohistone, (DNH), in various solvent conditions, together with gel electrophoresis studies of DNH modified with DTNB, sh...
متن کاملISOLATION AND PROPERTIES OF CRYSTALLINE pAPAIN*
Proteolytic enzymes from plant tissues have in general resisted purification more successfully than those of animal origin. Proteinases have been extracted from many plants, among them yeast (9), pineapple (27), pumpkin (27), beans,’ and wheat (4), but much concentration and purification are usually required before a preparation of even moderate activity is obtained. This presents difficulties ...
متن کاملcommuting and non -commuting graphs of finit groups
فرض کنیمg یک گروه غیر آبلی متناهی باشد . گراف جابجایی g که با نماد نمایش داده می شود ،گرافی است ساده با مجموعه رئوس که در آن دو راس با یک یال به هم وصل می شوند اگر و تنها اگر . مکمل گراف جابجایی g راگراف نا جابجایی g می نامیم.و با نماد نشان می دهیم. گرافهای جابجایی و ناجابجایی یک گروه متناهی ،اولین بار توسطاردوش1 مطرح گردید ،ولی در سالهای اخیر به طور مفصل در مورد بحث و بررسی قرار گرفتند . در ،م...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1958
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)70490-0